I'd skip the jumbo frames if I were you, makes bugger all difference to gigabit ethernet (10gbe is a different story) and just makes life hard, not many environments are setup for it and if you get it wrong you'll have all sorts of fun and games. Stick with the defaults which is probably an MTU of 1500.
When doing the copy make sure the 2003 and 2008 servers are part of the same domain and make sure you set the right options with Robocopy to copy over all the permissions and other ACL stuff.
Are you going to do a big bang migrate ? Or do a bulk copy then incremental copies before you cut over ?
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